Composite

Part:BBa_K824009:Experience

Designed by: Steven Allen   Group: iGEM12_Gaston_Day_School   (2012-10-02)

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Applications of BBa_K824009

User Reviews

UNIQ0d22ada0709980a2-partinfo-00000000-QINU UNIQ0d22ada0709980a2-partinfo-00000001-QINU

We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). We tested the detector with Cadmium since the promoter is responsive to multiple heavy metals. Due to safety issues, we were unable to use Arsenic because we are in BSL1 and it is a known poison. The results were measured in fluorescence per OD 600. The graph below shows our results.


Arsgraph.jpg